Analysis of Effect of Powder for Ascending and Descending on Treated or Initial Treatment Patient with Cough / 浙江中医药大学学报

Objective] To analyze the effect of powder for ascending and descending on patients with different cough. [Method] The treatment group patients had still cough with conventional western medicine for two weeks, and initial treatment group patients had not any treatment after cough. All these patients were treated with powder for ascending and descending according to different Chinese traditional medicine syndromes cough. [Results] There were 49 patients, and 17 cases were the initial diagnosed group and 32 patients were the after treatment group. Patients with the improvement of the symptoms of cough and throat were effective in 15 cases(88.2%), while in patients with the improvement for sputum symptoms in initial group were 16 cases(94.1%), and 27 case(84.4%) in treatment group. It had no statistically significant difference between the two groups(χ2=0.006, P=0.940). Cough concrete improvement between the two groups showed no significant difference(χ2 =0.006, P =0.465). Between the two groups cough, throat and sputum symptoms overall improvement rate was 87.8%(43/49), no significant difference between the two groups(P>0.05). [Conclusion] Regardless of the treatment patients or untreated patients, treatment with powder for ascending and descending had good effect in improvement of the symptoms of cough, and the improvement of throat and sputum was effective, too.

Effect of Huzhang on expression of lung fibroblasts' MMP-2/TIMP-1mRNA in pulmonary fibrosis rats / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effect of Huzhang on the progress of pulmonary fibrosis in rats, evaluate the role of Huzhang in this process and explore its mechanism.</p><p><b>METHOD</b>Wistar male rats were randomized into 7 groups (normal control group, model group, positive control group, prophylactic group, 3rd day treatment group, 7th day treatment group and 14th day treatment group). Bleomycin was administered by intratracheal injection to produce pulmonary fibrosis groups except the normal control group. The positive control group began to be given DXM (4 mL x kg(-1) x d(-1)) on the day of the model-making. The normal control group and model group were given NS (4 mL x kg(-1) x d(-1)) on the day of the model-making. The prophylactic group was given reagent (4 mL x kg(-1) x d(-1)) 2 days ahead of the model-making, whereas the 3rd day treatment group, the 7th day treatment group and the 14th day treatment group given the same dose respectively on the third day, the seventh day and the fourth day behind of the model-making. Lung tissue was stained with hematoxylin-eosin (HE) and Masson's trichrome to determine the pathological grading. The lung fibroblast (LF) was cultured in vitro by way of pancreatic enzyme digestion, which was used to detect the contents of the expression of MMP-2 and TIMP-1mRNA with RT-PCR method.</p><p><b>RESULT</b>Compared with those in the model group, the alveolitis, pulmonary fibrosis and collagen accumulation were significantly alleviated in the positive control group, Huzhang prophylactic group and each treatment groups. In the positive control group, Huzhang prophylactic group, the 3rd day treatment group, the 7th day treatment group and the 14th day treatment group, the expression of MMP-2 mRNA was weaker significantly than that in the BLM model group (P < 0.05 or P < 0.01) except that on the 42nd day. The expression of TIMP-1mRNA was also weaker significantly than that in the BLM model group at all set times in all treatment groups (P < 0.05 or P < 0.01). The inhibition of TIMP-1 lasted until the 42nd day.</p><p><b>CONCLUSION</b>Huzhang inhibited the expression of MMP-2mRNA and TIMP-1mRNA of lung fibroblast in different periods to reduce the alveolitis and pulmonary fibrosis, which was probably one of the anti-fulmonary fiborsis mechanisms of Huzhang.</p>

Study of Fanggan Decoction,s death prevention on mouse and inhibition effects on Influenza A virus / 中华中医药杂志

Objective:To study the Fanggan Decoction,s death prevention on mouse and inhibition effects on Influenza A virus in vivo.Methods:After setting up the model of mouse infected with Influenza A virus(H1N1),we observed the death prevention with Fanggan Decoction,done hemagglutination test and detected the dynamic contents of virus with Real-Time Fluorescence Quantitative RT-PCR.Results:Fanggan Decoction can prevent the death of infected mouse and delay the survival time.The death rate was 66.67%,33.33% and 25% respectively in low,middle and high dose of Fanggan Decoction groups and the average survival time was respectively 8.75 days,11.41 days and 12.33 days.Virus contents reached peak on the 5th day,while compared with the model group,virus contents were lower in each Fanggan Decoction groups,especial in the middle and high dose groups.Conclusion:Fanggan Decoction had good effect in inhibiting Influenza A virus,and can prevent the death of infected mouse,delay the survival time,while get better antivirus dose-effect relationship at double dose.

Treatment of Cough in line with Seasons’ Change of Climate in Inner Canon of Huangdi / 中华中医药杂志

Nei Jing says:Tian Qi connects Lung. Cough is closely associated with the seasons’change of climate which means four seasons and six pathogenic factors,so we must combine the clinical treatment of cough with the seasons’change of climate,which conduct an overall analysis of the illness and the patient's condition. It is favorable to drive out pathogenic Qi and restore healthy one.

Effect of Rhizoma Polygoni Cuspidati on expression of TGF-?_1 in rats with pulmonary fibrosis / 中华中医药杂志

Objective: To study the effects of Rhizoma Polygoni Cuspidati on expression of transforming growth factor beta 1(TGF-?1) in rats with pulmonary fibrosis induced by bleomycin.Method: 120 male SD rats were randomly divided into five groups: the control group(n=30),the bleomycin(BLM) model group(n=30),the Rhizoma Polygoni Cuspidati prophylactic group(n=30),the 7 th day treatment group(n=20) and the 28 th day treatment group(n=10).The rat models of pulmonary fibrosis were established by intratracheal injection of bleomycin, while the control group was given with normal saline instead.The Rhizoma Polygoni Cuspidati prophylactic group received intragastric administration with 4ml?kg-1?d-1 Rhizoma Polygoni Cuspidati 2 days before setting up models.The 7 th day treatment group and the 28 th day treatment group received the treatment on the seventh day and the twenty-eighth days after establishing models respectively.The other two groups received saline instead.On the 3rd,7th,14th,28th,42th and 56th day after instillation of bleomycin,lung samples were obtained and the content of hydroxyproline in lung tissue homogenate was detected to judge the degree of fibrosis and the therapeutic efficacy.At the same time the express of TGF-?1mRNA and TGF-?1 protein was examined by RT-PCR and immunohistochemical method.Results: Compared with the model group,the content of hydroxyproline in the Rhizoma Polygoni Cuspidati prophylactic group and the 7th day treatment group decreased significantly(P